Methods for Plant Molecular Biology

Methods for Plant Molecular Biology

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Methods for Plant Molecular Biology is a collection of articles that focuses on the techniques used in plant molecular biology and genetics. The book discusses the isolation and characterization of nuclear, chloroplast, and mitochondrial nucleic acids and the factors and systems involved in transcription and gene expression. Procedures for the isolation of cell walls, chloroplast membranes, membrane proteins; techniques to carry out plant cell culture and protoplast formation; and methods for gene and organelle transfer are covered as well. Biologists, molecular biologists, botanists, and students will find the book very useful.... The mtDNA is obtained from purified mitochondria isolated from leaf homogenates or from French Press ruptured cell cultures; ... Buffers Buffer A: 0.5 M sucrose, 5 mM EDTA (dibasic), 50 mM Tris-HCl, pH 7.5; add before use BSA ( Fraction V, anbsp;...

Title:Methods for Plant Molecular Biology
Author: Arthur Weissbach, Herbert Weissbach
Publisher:Elsevier - 2013-09-24

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